{"author_name":"Saskia Hiltemann","author_url":"https://galaxy.training","description":"Nanopore sequencing has several properties that make it well-suited for our purposes  1. Long-read sequencing technology offers **simplified** and less ambiguous genome **assembly** 2. Long-read sequencing gives the ability to **span repetitive genomic regions** 3. Long-read sequencing makes it possible to **identify large structural variations**  Open image in new tabFigure 1: Using nanopore sequencing, a single molecule of DNA or RNA can be sequenced without the need for PCR amplification or chemical labeling of the sample. (Image from: Nanopore sequencing: The advantages of long reads for genome assembly)    When using Oxford Nanopore Technologies (ONT) sequencing, the change in electrical current is measured over the membrane of a flow cell. When nucleotides pass the pores in the flow cell the current change is translated (basecalled) to nucleotides by a basecaller. A schematic overview is given in the picture above.  When sequencing using a MinIT or MinION Mk1C, the basecalling...","height":400,"html":"<iframe width=\"560\" height=\"400\" scrolling=\"yes\" sandbox=\"allow-same-origin allow-scripts\" title=\"FAQ: Nanopore sequencing\" src=\"https://training.galaxyproject.org/training-material/faqs/galaxy/sequencing_nanopore.html?utm_source=galaxy-help&utm_medium=oembed&utm_campaign=oembed\" frameborder=\"0\" allowfullscreen></iframe>","provider_name":"Galaxy Training Network (GTN)","provider_url":"https://galaxy.training","thumbnail_height":400,"thumbnail_url":"https://training.galaxyproject.org/training-material/assets/images/GTNLogo1000.png","thumbnail_width":560,"title":"FAQ: Nanopore sequencing","type":"video","version":"1.0","width":560}